FHI 360 and Sonke Gender Justice conducted a gender analysis for microbicide introduction in South Africa. The process included a desk review, interviews with key stakeholders, and an interpretation meeting to identify gender norms and inequalities that increase women’s HIV risk and could inhibit women’s access and adherence to microbicides in the future. This brief outlines 11 strategies that could be part of a microbicide introduction program to overcome these gender-related barriers.